RP-HPLC Method Development and Validation for Simultaneous Estimation of Alprazolam and Melatonin in Pharmaceutical Dosage Form

Simultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form

The present study describes a simple, accurate, precise and cost effective UV-Vis Spectroscopic and RP-HPLC method for the estimation of Ibandronate sodium (IBN). The determination of Ibandronate sodium (IBN) was performed by both UV and RP-HPLC method using 215 nm as the determination wavelength. The drug was dissolved in NaOH solution (0.1N NaOH) for estimation in UV and in distilled water fo...

Development and Validation of RP-HPLC method for simultaneous estimation of Methyldopa and Hydrochlorothiazide in Pharmaceutical Dosage Form

INTORDUCTION Methyldopa is (2S)-2-amino-3-(3, 4dihydroxyphenyl)-2-methylpropanoic acid (figure1a). Hydrochlorothiazide is 6-chloro-1, 1-dioxo-3,4dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide (figure1b). Methyldopa and Hydrochlorothiazide are official in Indian Pharmacopoeia and U.S. Pharmacopoeia, but there is no official method for the combination. Both drugs in combination of tablet dosage ...

Analytical Method Development for the Estimation of Alprazolam and Melatonin by Using Rp-hplc in Bulk and Tablet Dosage Form

A validated RP-HPLC method for simultaneous estimation of Dexrabeprazole and Domperidone in pharmaceutical dosage form

A validated reverse phase high performance liquid chromatography method has been developed for the simultaneous determination of Dexrabeprazole and Domperidone in combined dosage form. Chromatography was carried out on a C-18 column (4.6 mm × 250 mm, 5 μm) using Acetonitrile: 0.025 M potassium dihydrogen orthophosphate buffer (pH adjusted to 5.1 with triethylamine) in the ratio of 30:70 (v/v) a...

A Validated Rp-hplc Method for Simultaneous Estimation of Amlodipine and Lisinopril in Pharmaceutical Dosage Form

A reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of amlodipine and lisinopril in marketed formulation is developed. The determination was carried out on a Phenomenex C18 (250 x 4.6 mm, 5 μm) column using a mobile phase of 0.02 M phosphate buffer solution: methanol (75:25v/v, pH 7.0). The flow rate was 1.0ml/min with detection at 212 nm. The...